ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C.

Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA-1; Clonality Polyclonal; Validation KnockOut Validated

no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, … The purpose of this study was to verify the effect of berberine (BBR) on endoplasmic reticulum stress (ERS) and apoptosis of intestinal epithelial cells (IECs) in mice with ulcerative colitis (UC). BALB/c mice were randomly divided into five groups as follows: blank control, model, and low-, medium-, and high-dose BBR. A dextran sodium sulfate- (DSS-) induced model of UC was prepared, and the 2018-07-13 These PVDF membranes were incubated with the antibodies against Caspase‐3 (Abcam, 1:1000, ab13847), ATP7A (Abcam, 1:1000, ab13995), ATP7B (Abcam, 1:1000, ab124973), and β‐actin (Abcam, 1:1000, ab8227) overnight at 4°C and further incubated with HRP‐conjugated secondary antibody (1:6000). 2.10 Luciferase assay Fusion of caudal vertebrae has evolved multiple times independently: the pygostyle of birds, coccyx in apes and humans, ural plate of fish, and the urostyle of frogs. The anuran urostyle, however, is structurally and developmentally distinct because of the contribution of an ossifying hypochord. To date, the developmental mechanisms behind an ossifying hypochord have remained obscure.

Abcam. ab5369. WB. Anti-Sigma1. Santa Cruz. Sc22948. IF. Anti-CD3 (. Alexa Fluor®.

UniProt Code History.

Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) BioLegend 100209 IF Anti-CD86 (APC-conjugated) BioLegend 105011 FC Anti-CD11b (Alexa Fluor® 488-conjugated) BioLegend 101217 FC Anti-CD11c (FITC-conjugated) BioLegend 117305 FC Anti-CD4 (FITC-conjugated) BioLegend 100405 FC

Select a category from the list below in order to view or download the data. I 've been having some issues with detecting cleaved caspase 3, 7 and 9 in my w.blots. To elaborate, I am working with A375 (human melanoma), SQ20B (human squamous h&n carcinoma), HT1080 (human 2018-08-31 Anti-Caspase 9 Antibody, active (cleaved) form Chemicon®, from rabbit; find Sigma-Aldrich-AB3629 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems.

Mar 18, 2020 ab13847. WB. Anti-eIF2a. Abcam. ab5369. WB. Anti-Sigma1. Santa Cruz. Sc22948. IF. Anti-CD3 (. Alexa Fluor®. 647-conjugated). BioLegend.

If you have written or know of … I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview.

Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing the cancer-specific TNF-related apoptosis-inducing ligand for targeting and Maternal sevoflurane exposure during pregnancy is associated with increased risk for behavioral deficits in offspring. Several studies indicated that neurogenesis abnormality may be responsible for the sevoflurane-induced neurotoxicity, but the concrete impact of sevoflurane on fetal brain development remains poorly understood. dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight. After washing the cells with PBS, we incubated the cells with fluorescent secondary antibody dilution (Sigma-Aldrich, St. Louis, MO, USA) for 1 hour in the dark. We then washed the cells with PBS 2020-01-06 · Background Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide. Growing studies have proofed that circular RNAs (circRNAs) engage in the regulation of various types of cancers.
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Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunoprecipitation (IP) applications. After blocked with blocking buffer (P0260, Beyotime, China) for 10 min at room temperature, the slices were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam, USA), cleaved active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) BioLegend 100209 IF Anti-CD86 (APC-conjugated) BioLegend 105011 FC Anti-CD11b (Alexa Fluor® 488-conjugated) BioLegend 101217 FC Anti-CD11c (FITC-conjugated) BioLegend 117305 FC Anti-CD4 (FITC-conjugated) BioLegend Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB Equal amounts of proteins were separated by SDS-PAGE and transferred to a polyvinylidene fluoride membrane. After blocking for 1 h with 5% nonfat milk, the membranes were incubated overnight at 4 °C with Caspase-3 (Abcam, ab13847), Bcl-2 (Abcam, ab32124), Bcl-xl (Abcam, ab32370), PARP (Abcam, ab191217), and beta-actin (Proteintech, 66009). Apoptosis plays a key role in the pathogenesis of Parkinson disease (PD). Active caspase-3, which is a proapoptotic factor, has been shown to reduce cardiac contractility, causing cardiac dysfunction in many pathological diseases.

Breast cancer is a growing Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)–loaded EVs (IL-10+ EVs) by engineering macrophages for treating ischemic acute kidney injury (AKI). Delivery of IL-10 via EVs enhanced not only the stability of IL-10, but also its targeting to the kidney 2019-03-12 · The cells were counted in five random fields per well. For BMSCs grafts evaluation, rats were sacrificed at 7 days after transplantation and the femoral trochlea were harvested.
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BAK (D4E4, #12105), and Hsp60 (#4870); from Abcam: active caspase 3 (#ab13847), BCL-xL (#ab32370, used at 1/500 dilution), BCL-2 (#ab18210, used at

We report an approach to manufacturing interleukin-10 (IL-10)–loaded EVs (IL-10+ EVs) by engineering macrophages for treating ischemic acute kidney injury (AKI).

2020-10-21

Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data. Current treatment for glioblastoma multiforme (GBM) fails to address its highly infiltrative nature; treatment often leaves behind microscopic neoplastic satellites, resulting in eventual tumor recurrence. Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing the cancer-specific TNF-related apoptosis-inducing ligand for targeting and Maternal sevoflurane exposure during pregnancy is associated with increased risk for behavioral deficits in offspring. Several studies indicated that neurogenesis abnormality may be responsible for the sevoflurane-induced neurotoxicity, but the concrete impact of sevoflurane on fetal brain development remains poorly understood. dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight.

Antibody 2021-02-08 · After blocked with blocking buffer (P0260, Beyotime, China) for 10 min at room temperature, the slices were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam, USA), cleaved 2020-01-30 · 1. Introduction.